S-layer

An S-layer (surface layer) is a part of the cell envelope commonly found in archaea, as well as bacteria.^[1][2] It consists of a monomolecular layer composed of identical proteins or glycoproteins. This structure is built via self-assembly and encloses the whole cell surface. Thus, the S-layer protein can represent up to 10–15% of the whole protein content of a cell.^[3][4][5] S-layer proteins are poorly conserved or not conserved at all, and can differ markedly even between related species. Depending on species, the S-layers have a thickness between 5 and 25 nm and possess identical pores with 2–8 nm in diameter.^[6] S-layers exhibit either an oblique (p1, p2), square (p4) or hexagonal (p3, p6) lattice symmetry. Depending on the lattice symmetry, the S-layer is composed of one (P1), two (P2), three (P3), four (P4), or six (P6) identical protein subunits, respectively. The center-to-center spacings (or unit cell dimensions) between these subunits range between 2.5 and 35 nm.

Gram-Positive Cell Envelope showing the location of S-layer proteins.

Gram-Negative Cell Envelope showing the location of S-layer proteins.

Fixation of S-layers in the cell wall

• In Gram-negative bacteria, S-layers are associated to the lipopolysaccharides via ionic, carbohydrate–carbohydrate, protein–carbohydrate interactions and/or protein–protein interactions.
• In Gram-positive bacteria whose S-layers often contain surface layer homology (SLH) domains, the binding occurs to the peptidoglycan and to a secondary cell wall polymer (e.g., teichoic acids). In the absence of SLH domains, the binding occurs via electrostatic interactions between the positively charged N-terminus of the S-layer protein and a negatively charged secondary cell wall polymer.
• In Gram-negative archaea, S-layer proteins possess a hydrophobic anchor that is associated with the underlying lipid membrane.
• In Gram-positive archaea, the S-layer proteins bind to pseudomurein or to methanochondroitin.

Biological functions of the S-layer^[7] For many bacteria, the S-layer represents the outermost interaction zone with their respective environment. Its functions are very diverse and vary from species to species. In many archaeal species the S-layer is the only cell wall component and, therefore, is important for mechanical and osmotic stabilization. Additional functions associated with S-layers include:

• protection against bacteriophages, Bdellovibrios, and phagocytosis
• resistance against low pH
• barrier against high-molecular-weight substances (e.g., lytic enzymes)
• adhesion (for glycosylated S-layers)
• stabilisation of the membrane
• provision of adhesion sites for exoproteins
• provision of a periplasmic compartment in Gram-positive prokaryotes together with the peptidoglycan and the cytoplasmic membranes

S-layer structure

While ubiquitous among Archaea, and common in bacteria, the S-layers of diverse organisms have unique structural properties, including symmetry and unit cell dimensions, due to fundamental differences in their constituent building blocks. Sequence analyses of S-layer proteins have predicted that S-layer proteins have sizes of 40-200 kDa and may be composed of multiple domains some of which may be structurally related. Since their discovery in the 1950s^[8] S-layer structure has been investigated extensively by electron microscopy and medium resolution images of S-layers from these analyses has provided useful information on overall S-layer morphology. High-resolution structures of an archaeal S-layer protein (MA0829 from Methanosarcina acetivorans C2A) of the Methanosarcinales S-layer Tile Protein family and a bacterial S-layer protein (SbsB), from Geobacillus stearothermophilus PV72, have recently been determined by X-ray crystallography.^[9][10] In contrast with existing crystal structures, which have represented individual domains of S-layer proteins or minor proteinaceous components of the S-layer, the MA0829 and SbsB structures have allowed high resolution models of the M. acetivorans and G. stearothermophilus S-layers to be proposed. These models exhibit hexagonal (p6) and oblique (p2) symmetry, for M. acetivorans and G. stearothermophilus S-layers, respectively, and their molecular features, including dimensions and porosity, are in good agreement with data from electron microscopy studies of archaeal and bacterial S-layers.

References